Antibody peptide and small molecule labeling

Development of novel radiolabeled tracers for in vivo targeting. Proteins (antibodies), peptides, small molecules and liposomes are labelled with various radionuclides such as 111In, 68Ga, 125I, 131I, 99mTc, 213Bi, 177Lu, 18F, for PET imaging, SPECT imaging or therapy. After successful production of the radiotracers, extensive in vitro characterization is performed. In vitro characterization includes stability tests and affinity and internalization characteristics using cells expressing the target of interest. The newly developed tracers are consecutively characterized for imaging and therapy studies in the field of oncology, diabetes, rheumatoid arthritis and neurology.


People

Funding

  • European Union

Publications

  • S. Heskamp, W. Hobo, J.D. Molkenboer-Kuenen, D. Olive, W.J.G. Oyen, H. Dolstra and O.C. Boerman. "Non-invasive imaging of tumor PD-L1 expression using radiolabeled anti-PD-L1 antibodies", Cancer Research 2015;75:2928-2936. Abstract/PDF DOI PMID

  • M. Brom, L. Joosten, W.J. Oyen, M. Gotthardt and O.C. Boerman. "Improved labelling of DTPA- and DOTA-conjugated peptides and antibodies with 111In in HEPES and MES buffer", EJNMMI Res 2012;2:4. Abstract/PDF DOI PMID

  • S. Heskamp, P. Laverman, D. Rosik, F. Boschetti, W.T.A. van der Graaf, W.J.G. Oyen, H.W.M. van Laarhoven, V. Tolmachev and O.C. Boerman. "Imaging of human epidermal growth factor receptor type 2 expression with 18F-labeled affibody molecule ZHER2:2395 in a mouse model for ovarian cancer", Journal of Nuclear Medicine 2012;53:146-153. Abstract/PDF DOI PMID

  • M. Brom, L. Joosten, P. Laverman, W.J.G. Oyen, M. Béhé, M. Gotthardt and O.C. Boerman. "Preclinical evaluation of 68Ga-DOTA-minigastrin for the detection of cholecystokinin-2/gastrin receptor-positive tumors", Molecular Imaging 2011;10:144-152. Abstract PMID

  • M. Brom, W.J.G. Oyen, L. Joosten, M. Gotthardt and O.C. Boerman. "68Ga-labelled exendin-3, a new agent for the detection of insulinomas with PET", European Journal of Nuclear Medicine and Molecular Imaging 2010;37:1345-1355. Abstract/PDF DOI PMID

  • S. Heskamp, H.W.M. van Laarhoven, J.D.M. Molkenboer-Kuenen, G.M. Franssen, Y.M.H. Versleijen-Jonkers, W.J.G. Oyen, W.T.A. van der Graaf and O.C. Boerman. "ImmunoSPECT and immunoPET of IGF-1R expression with the radiolabeled antibody R1507 in a triple-negative breast cancer model", Journal of Nuclear Medicine 2010;51:1565-1572. Abstract/PDF DOI PMID